plasmid prep

plasmid prep
Generic term for the isolation of recombinant plasmids from liquid bacterial culture, usually by alkaline/detergent lysis, selective precipitation of other components, and affinity purification of plasmid. As this is the most exciting thing most molecular biologists ever do, there is an informal shorthand for the scale of the preparation based on the size of the overnight culture: see miniprep, midiprep, maxiprep and megaprep.

Dictionary of molecular biology. 2004.

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Look at other dictionaries:

  • Prep — may refer to: * P rep, a statistical value of the probability of replicating an observed effect * Prep , a novel by Curtis Sittenfeld * Prep, short form for a plasmid preparation (including minipreps and bulk preps) or the DNA prepared by such a… …   Wikipedia

  • Plasmid — Figure 1: Illustration of a bacterium with plasmid enclosed showing chromosomal DNA and plasmids. In microbiology and genetics, a plasmid is a DNA molecule that is separate from, and can replicate independently of, the chromosomal DNA …   Wikipedia

  • mini-prep — A small scale preparation of plasmid or phage DNA. Used to purify cloned DNA from the DNA of the vector …   Glossary of Biotechnology

  • Promega — History and Background =In 1978 scientists were just beginning to understand the potential of what Boyer and Cohen had done by cutting DNA into specific fragments using restriction enzymes, rejoining them and transferring them into bacterial host …   Wikipedia

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